Owing to the technique’s high sensitivity towards large species, even small impurities caused by aggregation can be detected Owing to its large measurement range (0.5 nm – 1 µm) DLS closes the gap between the range of small soluble aggregates that are detected via size exclusion chromatography (SEC) and particles analyzed by methods such as light obscuration (LO) or micro flow imaging (MFI). ZentriForce Pharma offers DLS services under BSL 1 and 2.ĭynamic light scattering is known as a fast and sensitive screening method for aggregate / particle detection and therefore widely used within biopharmaceutical development. We routinely conduct biosimilarity and gene therapy studies and are furthermore experienced in the development of dynamic light scattering (DLS) methods for a large variety of samples. Zentriforce Pharma is a contract research organization that specializes in analytical services with a focus on hydrodynamic methods. Asymmetrical Flow Field-Flow Fractionation (AF4) Theory.Sedimentation Equilibrium Analytical Ultracentrifugation (SE-AUC).Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) Data Evaluation Approaches.Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC).DLS Services for the Development of other Gene Therapy APIs.AF4 Services for the Development of other Gene Therapy APIs.SV-AUC Services for the Development of other Gene Therapy APIs.SEC-MALLS Services for Peptide Development.SV-AUC Services for Peptide Development.SEC-MALLS Services for Biopharmaceutical Development.DLS Services for Biopharmaceutical Development.AF4 Services for Biopharmaceutical Development.SV-AUC Services for Biopharmaceutical Development.SEC-MALLS Services for Biosimilarity Studies.SV-AUC Services for Biosimilarity Studies.Dynamic Light Scattering (DLS) Services.Differential Scanning Calorimetry (μ-DSC) Services.Size Exclusion Chromatography (SEC-MALLS) Services.Asymmetrical Flow Field-Flow Fractionation (AF4) Services.Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) Services.After the recalculation, consistent values of mean particle diameter are found to be between those obtained by DLS and DMA within the estimated uncertainties. Before the comparison, the intensity-averaged size measured by DLS is recalculated to the number-averaged size in the case of DMA from the particle size distribution. The extrapolated particle size measured by DLS is compared to the size determined by a differential mobility analyzer (DMA) in air. The extrapolations of apparent diffusion coefficients to infinite dilution and to lower angles yield more precise values than those obtained at one angle and one concentration. Precise measurements of the short time correlation function at seven scattering angles and five different concentrations are performed for four kinds of polystyrene latex suspensions with diameters of 30–100 nm. However, the precision of this technique remains unclear and therefore, in the current work, the precision of the DLS technique for sizing polystyrene latex suspensions and the uncertainty of the DLS data are estimated. Dynamic light scattering (DLS) is a powerful technique for the sizing of nanoparticles and characterization of their properties in the liquid phase.
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